G-Rex ® stands for Gas Permeable Rapid Expansion and is the result of applying our disruptive technology to the needs of immunotherapy. G-Rex ® is the only production platform created specifically to produce immune cells such as T cells, natural killer cells, and hematopoietic cells. G-Rex® is proving to be the most practical and cost effective way …
Large-scale expansion of V?9V?2 T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor-modified effector cells Cytotherapy . 2018 Mar20(3):420-435. doi: 10.1016/j.jcyt.2017.12.014.
aAPCs in G-Rex cell culture vessels forV?9V?2T-cell expansion.A two-step protocol was developed in which PBMCs were ?rst activated with Zometa and interleukin (IL)-2 for 7 days and the activated cells were then numerically expanded by co-culturing with K562-aAPCs, anti-human CD3 monoclonal anti-body OKT3, Zometa and IL-2 in G-Rex cell culture, 12/1/2019 · The G-Rex has been shown to be effective in expanding tumor-infiltrating lymphocytes (TILs) [5,6], natural killer (NK) cells , , , V?9V?2 T cells , regulatory T cells and virus-specific T cells (VSTs) , , , . In the context of genetically modified cells, such as CAR T cells, G-Rex bioreactors do not directly address the added complexity of gene transfer, however, viral transduction in solution with Vectofusin.
In the G-Rex platform, examples of cell expansion (9–14 day duration) include CAR- T cells , which have atypical harvest density of 20–30 × 10?/cm² (or 2–3 × 10? cells in a 100 cm² …
G-REX ® DOCUMENTATION | Wilson Wolf Manufacturing, G-REX® FOR T CELL THERAPY | Wilson Wolf Manufacturing, G-REX ® DOCUMENTATION | Wilson Wolf Manufacturing, G-REX ® DOCUMENTATION | Wilson Wolf Manufacturing, In the G-Rex platform, examples of cell expansion (9-14 day duration) include CAR-T cells, which have atypical harvest density of 20-30 × 10 6 /cm 2 (or 2-3 × 10 9 cells in a 100 cm 2 device) NK cells, which have a typical harvest density of 20-30 × 10 6 /cm 2 (or 2-3 × 10 9 cells in a 100 cm 2 device) and numerous other cell types that proliferate without the need for intervention or complex processes.
9/1/2020 · Streamlined production of genetically modified T cells with activation, transduction and expansion in closed-system G-Rex bioreactors. “This study demonstrates that T cells can be transduced with retroviral vectors in solution in the G-Rex bioreactor with addition of Vectofusin-1.
We have previously demonstrated that this G-Rex platform not only supports the expansion of antigen-specific and genetically modified T cells and a range of suspension cell lines but also produces superior cell output (3- to 20-fold increase in cell numbers) with a reduction in the number of required technician interventions (?4-fold) when compared with conventional 24-well plates and flasks.
• 2Cell expansion typically ends when cells are at between 10 -30 million cells per cm . • G-Rex10 and G-Rex100 are typically fed only once every 5 days so long as cytokines (typically IL- 2) are added at the same frequency as your standard protocols require (typically every 2-3
